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Recent studies have demonstrated that arsenic at low concentrations is a potent inducer of colon cancer. The mechanism of arsenic-induced carcinogenesis is not fully understood. In this study, we compared the effects of arsenic treatment alone and in combination with l-buthionine-(S,R)-sulfoximine (BSO) on the proliferation of HT29 cells and the activity of glutathione S-transferases (GST). The results demonstrate that both arsenite and arsenate induce the proliferation of HT29 cells. However, the addition of BSO to cells previously exposed to arsenic failed to stimulate cell proliferation. We found that arsenite and arsenate treatments caused a significant increase in both GST activity and GST-mu. In contrast, the combination of BSO and arsenic caused a decrease in both GST activity and GST-mu. These results demonstrate that arsenic treatment induces cell proliferation and expression of GST-mu, an enzyme that plays an important role in the detoxification of electrophilic and carcinogenic compounds. Our results also indicate that BSO can prevent the arsenic-induced increase in GST activity, a finding which is consistent with the hypothesis that arsenic-induced changes in the glutathione system contribute to the initiation of tumorigenesis.---------------------- Forwarded by Mike McConnell/HOU/ECT on 05/25/2001 07:36 PM ------------------------ What's New In? System Requirements: Languages: English SteamOS: Linux - 0.7.1-1 Minimum recommended GPU: GPU: (depends on the game) NVIDIA GeForce 320 or newer (2GB of VRAM) AMD Radeon R7 or newer (2GB of VRAM) Intel HD 4000 or newer Intel HD 5xxx or newer Recommended: NVIDIA GeForce 330 or newer (2GB of VRAM) AMD Radeon R9 or newer (2GB of VR
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