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Recent studies have demonstrated that arsenic at low concentrations is a potent inducer of colon cancer. The mechanism of arsenic-induced carcinogenesis is not fully understood. In this study, we compared the effects of arsenic treatment alone and in combination with l-buthionine-(S,R)-sulfoximine (BSO) on the proliferation of HT29 cells and the activity of glutathione S-transferases (GST). The results demonstrate that both arsenite and arsenate induce the proliferation of HT29 cells. However, the addition of BSO to cells previously exposed to arsenic failed to stimulate cell proliferation. We found that arsenite and arsenate treatments caused a significant increase in both GST activity and GST-mu. In contrast, the combination of BSO and arsenic caused a decrease in both GST activity and GST-mu. 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